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KMID : 0363320030240010113
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Journal of Korean Oriental Internal Medicine
2003 Volume.24 No. 1 p.113 ~ p.122
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The effect of Gagamchunggan-tang on lipopolysaccharide-induced expression of NF{kappa}-B downstream genes in HepG2 cell
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Kim Sung-Hwan
Seo Sang-Ho
Hong Sang-Hoon
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Abstract
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Objective : The aim of this study was to evaluate the efficacy of Gagamchunggan-tang on anti-inflammation reaction with lipopolysaccharide (LPS)-induced HepG2 cell.
Method : We examined the effects of the Gagamchunggan-tang, a traditional drug for liver inflammation, on the process of lipopolysaccharide(LPS)-induced nuclear factor-{kappa}Bp65(NF-{kappa}Bp65) activation in HepG2 cell. SDS-PAGE, Western blotting, Immunofluorescence staining were studied.
Results : Immunoblot analysis showed that the level of nucleic NF-{kappa}Bp65 was rapidly up-regulated and cytosolic inhibitory I-{kappa}B{alpha} was down-regulated by LPS challenge. While Gagamchunggan-tang inhibited an increase of NF-{kappa}Bp65 and degradation of I-{kappa}B{alpha} in HepG2 cell. Besides LPS-induced expression of a group of genes, such as tumor necrosis factor-{alpha}(TNF-{alpha}), inducible nitric oxide synthase(iNOS) and cyclooxygenase-2 (COX-2), are repressed by Gagamchunggan-tang. It may be concluded that Gagamchunggan-tang attenuates the progress of LPS-induced inflammation by reduction of NF-{kappa}Bp65 activation.
Conclusion : The Gagamchunggan-tang would be useful as a therapeutic agent for endotoxin-induced liver disease.
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KEYWORD
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Gagamchunggan-tang, LPS, NF-{kappa}Bp65
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